Journal: Cell reports

Article Title: Sterols lower energetic barriers of membrane bending and fission necessary for efficient clathrin-mediated endocytosis

doi: 10.1016/j.celrep.2021.110008

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Briefly, a guide RNA (5′-GCAGATGTAGTGTTTCCACA-3′) targeting the open reading frame in the immediate vicinity of the stop codon was cloned into the Cas9 expression vector pX330-U6-Chimeric_BB-CBh-hSpCas9 (gift from Feng Zhang; ), Addgene plasmid #42230).

Techniques: Derivative Assay, Recombinant, Electron Microscopy, Transfection, Expressing, Plasmid Preparation, Software, Cell Analysis, Gas Chromatography, Mass Spectrometry

Journal: Communications Biology

Article Title: CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding

doi: 10.1038/s42003-019-0288-7

Figure Lengend Snippet: Regeneration of genome-edited events by delivering CRISPR/Cas9 construct through Agrobacterium -mediated transformation of cell suspension of Gonja Manjaya. a Schematic representation of T-DNA region of binary vector pMR215-BSV1 showing the positions of three gRNAs. LB, left border; RB, right border; PAM, protospacer adjacent motif. Sequences of the gRNAs are also indicated. b and c Germination of Agro -infected embryos in selective medium containing kanamycin. d Well-rooted plantlets in proliferation medium. e Genome-edited Gonja Manjaya plants in glasshouse. f Non-edited wild type control Gonja Manjaya plants in glasshouse

Article Snippet: Then, all the three gRNAs were combined into one destination vector pDe-CAS9-Kan using Gateway LR II Plus clonase reaction (ThermoFisher) to yield the final plasmid pMR215-BSV1.

Techniques: CRISPR, Construct, Transformation Assay, Plasmid Preparation, Infection